Cryoprotectant toxicity and hypothermic sensitivity among Anopheles larvae
نویسندگان
چکیده
منابع مشابه
Cryoprotectant toxicity neutralization.
Cryoprotectant toxicity is a fundamental limiting factor for the successful cryopreservation of living systems by both freezing and vitrification, and the ability to negate it would be attractive. Past attempts to demonstrate "cryoprotectant toxicity neutralization" (CTN) have had many ups and downs. First convincingly introduced by Baxter and Lathe in 1971, the concept that certain amides can ...
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15 صفحه اولCryoprotectant Toxicity: Facts, Issues, and Questions
High levels of penetrating cryoprotectants (CPAs) can eliminate ice formation during cryopreservation of cells, tissues, and organs to cryogenic temperatures. But CPAs become increasingly toxic as concentration increases. Many strategies have been attempted to overcome the problem of eliminating ice while minimizing toxicity, such as attempting to optimize cooling and warming rates, or attempti...
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متن کاملPermeation and toxicity of ethylene glycol and methanol in larvae of Anopheles gambiae.
In this study, we applied proton NMR to measure the permeation of two cryoprotective agents (CPAs), ethylene glycol (EG) and methanol, into 1st instar Anopheles larvae. Calibration with standard solutions of EG or methanol (0-10 mol l(-1)) confirmed the reliability of the NMR measurements for determining the concentration of these solutes. To assess permeation, larvae were immersed in 1.5 mol l...
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ژورنال
عنوان ژورنال: Cryobiology
سال: 2021
ISSN: 0011-2240
DOI: 10.1016/j.cryobiol.2020.12.007